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Hotel Booking       Mahidol University Annual Research Abstracts 2000 421 STARCH BRANCHING ENZYME FROM CASSAVA TUBERS Nopphadol Aroonrungsawasdi¹, Tipaporn Limpaseni¹ and Montri Chulavatnatol^{2 1}Department of Biochemistry, Faculty of Science,Chulalaongkorn University; ²Department of Biochemistry, Faculty of Science, Mahidol University.

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Cheap Hotel Key words : starch branching enzyme, SBE, cassava

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Travel Agent                      Starch branching enzyme (SBE, also called Q-enzyme) is involved in the synthesis of amylopectin, a component of starch. SBE plays important role in determining starch quality and quantity. In this report SBE is isolated from cassava tuber extract by polyethyleneglycol (PEG) fractionation followed by ion exchange chromatography on DEAE-cellulose and Q-Sepharose column. SBE activity was detected in both unbound and bound fractions from DEAE-cellulose column. The bound fractions were eluted at 0.09 M NaCl. The bound fractions were pooled and subjected to Q-Sepharose column. Two peaks containing SBE activity were detected in the bound fraction eluted at 0.06 and 0.16 M NaCl with purification of 120 and 176 folds respectively.

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Online Booking (Supported by National Science Technology and Development Agency. Presented at the 25^th Congress on Science and Technology of Thailand, 20-22 October, 1999 at Amarin Lagoon Hotel, Pitsanuloke.)   CHARACTERISTICS OF SUCROSE TRANSPORT IN CASSAVA LEAVES Thidarat Eksittikul¹, Montri Chulavatnatol² and Tipaporn Limpaseni^{3 1}Department of Biotechnology, Faculty of Science, Ramkhamhaeng, University, Bangkok 10320.²Department of Biochemistry, Faculty of Science, Mahidol University Rama 6 Road, Bangkok, 10400. ³Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330.

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Hotel Reservation Keywords: sucrose transport, cassava, ¹⁴C-sucrose

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Discount Travel                      Sucrose is a form of assimilated carbon derived from photosynthesis, and must be transport to non-photosynthetic organs for its growth, development, germination, seedling, flowering and storage. Sucrose has been shown to be actively transported across cell membrane by transport protein. Sucrose transport in cassava leaves was studied by using uptake experiment. Leafdiscs of cassava (12 mm. diameter) was incubated in the medium containing ¹⁴C-sucrose and it was found that the sucrose uptake was linear with time and exhibited Michaelis Menten characteristics with the K^m value 5 mM and V_max value 13.33 nmol/disc/h. Sucrose uptake was inhibited by DNP suggesting the involvement of proton transport and the sucrose transport protein of cassava leaf contains Thio groups. Linamarin, the major cyanogenic glucoside in cassava can compete the sucrose uptake upto 50% at the concentration range 0.1 mM.

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Booking Agency (Supported by National Science Technology and Development Agency. Presented at the 25^th Congress on Science and Technology of Thailand, 20-22 October, 1999 at Amarin Lagoon Hotel, Pitsanuloke.)

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